Diagnosis of the presence of Badnavirus in banana plantations in the Province of El Oro
Abstract
Banana Streak Virus (BSV), belonging to genus Badnavirus, causes great yield losses in banana. It is an infectious agent that spreads through the seeding of infected material and through mealybugs (Pseudococcidae). It is responsible for significant losses in profitability and a serious constraint in the genetic improvement of Musa sp. The absence of a highly sensitive and specific technique to identify the affected plants has enabled it to be disseminated in the plantations. Therefore, to achieve a correct identification of the plants affected by Badnavirus the following objective was proposed: Diagnose the presence of Badnavirus in the banana plantations of the province of El Oro. Total Nucleic Acid Extracts of banana were obtained with 2% CTAB with which a suitable template DNA was obtained for the analysis of the polymerase chain reaction. Using a pair of primers that flank a region of 221 bp that corresponds to the gene of the reverse transcriptase and RNase H of the ORF 3 of the badnaviruses. With the use of this methodology, it was possible to determine the existence of this virus in 89.36% of the plantations evaluated.Downloads
References
Agrios, G. (1998). Fitopatología. Mexico: Limusa.
Armijos, M. (2004). Ciclo de vida de los piojos harinosos (cochinillas harinosas) del Banano y plátano en el Ecuador. Guayaquil: inIAP-PROMSA.
Braithwaite, K. E. (1995). Detection of sugarcane bacilliform virus using the polymerase chain reaction. Plant Disease.
Caruana, m. L. (1998). Activities of CIRAD virus indexing centre. Montpellier: INIBAP.
Curtis, H. B. (2001). Biología (6 ed.). España: Medica Panamericana.
Dahal, G. O. (2000). Relationship between natural occurrence of banana streak virus and symptom expression, relative concentration of viral antigen, and yield characteristics of some micropropagated Musa spp. Plant Pathology., Vol. 49.
Dahal, G. P. (1998). Studies on a Nigerian isolateof banana streak badnavirus: Effect of intraplanr variation on virus accumulation and reliability of diagnosis by ELISA . Ann. Appl. Biol, Vol. 132.
Daniells, J. G. (2001). The effect of banana streak virus on the growth and yield of dessert bananas in tropical Australia. Ann. Appl. Biol., Vol. 139.
Daniells, J. T. (1999). Banana streak disease. An ilustrated field guide. Australia: : Department of primary industries.
Diekmann, M. (1996). Technical guidelines for the safe movement of germplasm (2 ed.). Rome: IPGRI, Ed.
Dietzgen, R. T. (1999). PCR-based detection of viruses in banana and sugarcane. Current topics in Virology, Vol. 1.
Ferreira, M. (1998). Introducción al uso de marcadores moleculares en el análisis genético. Brasilia: EMBRAPA.
Frison, E. S. (1998). The economic, social and nutritional importance of banana in the world. Cameroon.
Garcia, M. R. (2012). Viral Genomes - Molecular Structure, Diversity, Gene Expression Mechanisms and Host-Virus Interactions. . InTech.
Harper, G. H. (1998). Cloning and sequence analysis of banana Streak Virus DNA. . Virus Genes, Vol. 17.
Harper, G. H. (2002). Detection of banana streak virus in field samples from Uganda. . Ann. Appl. Biol., Vol. 141.
Harper, G. H. (2002). Detection of banana streak virus in field samples from Uganda. . Ann. Appl. Biol., Vol. 141.
Harper, G. H. (2004). Banana streak virus is very diverse in Uganda . Virus Research, Vol. 100.
Harper, G. O.-H. (1999). Integration of Banana streask virus into the Musa genome: Molecular and citogenetic evidence. . Virology, Vol. 255.
Hernandez, P. A. (2011). Polymerase Chain Reaction:Types, Utilities and Limitations. intechopen, Ed.
Jones, D. (2000). Diseases of Banana, Abaca and Enset (Vols. Introduction toof Banana, Abaca and Enset. Worcestershire: C. publishing, Ed.
Jones, D. (2000). Diseases caused by viruses. Banana streak. Worcestershire: CABI publishing.
Khumallambam, D. K. (2013). An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications. Springer.
King, A. A. (2012). Virus taxonomy: Ninth report of the International Commitee on taxonomy of viruses. New York: Elsevier.
Kumar, L. S. (2014). Biology, Etiology, and Control of Virus Diseases of Banana and Plantain. . Elsevier.
Lassoudière. (1979).
Li, Q. C. (1994). A DNA extraction method for RAPD analysis from plants rich in soluble polysacharides. Plant Molecular Biology., Vol. 123.
Lockhart, B. (1993). Serological and genomic heterogeity of banana streak badnavirus: implications for virus detections in Musa germplasm. . Montpellier: CIRAD- FLHOR.
Lockhart, B. (1995). Banana streak badnavirus infection in Musa: Epidemiology, diagnosis and control. Taipei: F. a. Technology, Ed.
Lockhart, B. (1998). Management of viral sreak in banana and plantain: Understandong a new challenge. Davao: (INIBAP, Ed.
Lockhart, B. (1999). Badnaviruses.
Lodish, H. B. (2002). . (). Biología celular y molecular (4 ed.). España: Editorial Medica Panamericana.
Muylle, H. (1998). Development of techniques for the elimination of virus diseases from Musa:Progress Report. . Montpellier: INIBAP.
Naidu, R. H. (2000). Methods for the detection of plant virus diseases. Plant virology in sub-Saharan Africa.
Ndowora, T. D. (1999). Evidence that badnavirus infection in Musa can originate from integrated pararetroviral sequences. Virology, Vol. 255.
Pulimamidi, R. N. (2012). Gel-Electrophoresis and Its Applications. Hyderabad: Intechopen, Ed.
Zhang, Y. U. (1998). A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virological Methods., Vol. 71.
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