In vitro effect of fungicides for the control of Colletotrichum SPP. In fruit trees Manabí - Ecuador
DOI:
https://doi.org/10.29076/issn.2528-7737vol14iss35.2021pp37-42pKeywords:
anthracnose, disease, fungiAbstract
Anthracnose caused by Colletotrichum spp causes one of the most limiting diseases in the production of Passiflora edulis and Carica papaya in the province of Manabí, Ecuador. The objective of this research was to determine the pathogenicity of Colletotrichum isolates in passion fruit and papaya fruits, as well as their in vitro sensitivity to fungicides. The experiments were carried out in the Molecular Biology Laboratory of the Escuela Superior Politécnica Agropecuaria de Manabí. The healthy fruits of passion fruit, variety INIAP 2009 and papaya variety Hawaiian, were inoculated by collecting fungal mycelium fragments with the tip of a sterilized wooden stick. For the in vitro sensitivity of fungicides, a completely randomized experimental design was used, 8 commercial fungicide treatments, with 4 repetitions and the comparison of means was carried out with the Tukey test (P <0.05). All the isolates were pathogenic in passion fruit and papaya respectively, they caused characteristic anthracnose symptoms 4 to 5 days after inoculation. The fungicides tebuconazole, propiconazole, difenoconazole, benomyl, carbendazim and azoxystrobin totally inhibited the mycelial growth of the fungus, while chlorothalonil and copper sulfate partially decreased the development of Colletotrichum spp. Systemic fungicides were shown to be effective in the in vitro control of Colletotrichum spp. Isolated from passion fruit and papaya.
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