Extraction and quantification of polyphenols in Plantago major L. and Buddleja globosa
DOI:
https://doi.org/10.29076/issn.2602-8360vol9iss16.2025pp140-145pKeywords:
polyphenols, Plantago major L., Buddleja globose, macerationAbstract
Antioxidants play an important role in disease prevention. Various studies have shown that plants and their extracts are effective in protecting against damage caused by free radicals. It has been demonstrated that species such as Plantago and Buddleja exhibit antioxidant activity due to the presence of phenolic compounds. This study aimed to determine the influence of plant material on the concentration of polyphenols present in plant extracts. Leaves and roots of Buddleja and Plantago were collected, and 70% ethanol was used as a solvent in the maceration extraction method. The total polyphenol concentration was determined using the Folin-Ciocalteu method. Through analysis of variance, it was found that both the plant and its parts had a significant effect on the polyphenol concentration, with the extract from Buddleja leaves showing the highest polyphenol content (15.930 ± 1.375 mg GAE/g DW). The total flavonoid content was determined using the colorimetric method with aluminum chloride, corresponding to the extract from Buddleja leaves (2.577 ± 0.025 mg QE/g DW). Antioxidant capacity was determined using the DPPH method, with an equivalent concentration of Trolox of 12.463 ± 0.067 µmol/g.
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References
1. Castillejo N, Martínez-Zamora L. Bioactive Compounds from Fruit and Vegetable Waste: Extraction and Possible Utilization. Foods. 2024;13(5):775. doi: 10.3390/foods13050775
2. Repo de Carrasco, Ritva, Encina Zelada, Christian René. Determinación de la capacidad antioxidante y compuestos bioactivos de frutas nativas peruanas. Revista de la Sociedad Química del Perú. 2008;74(2), 108-124.
3. Esmaeili S, Dayani L, Taheri A, Zolfaghari B. Phytochemical standardization, formulation and evaluation of oral hard gelatin capsules from Pinus eldarica bark extract. Vol. 11.
4. Armijos C, Ramírez J, Salinas M, Vidari G, Suárez AI. Pharmacology and Phytochemistry of Ecuadorian Medicinal Plants: An Update and Perspectives. Pharmaceuticals. 2021;14(11):1145. doi: 10.3390/ph14111145
5. Salem O, Szwajkowska-Michałek L, Przybylska-Balcerek A, Szablewski T, Cegielska-Radziejewska R, Świerk D, et al. New Insights into Bioactive Compounds of Wild-Growing Medicinal Plants. Applied Sciences. 2023;13(24):13196. doi: 10.3390/app132413196
6. Hikal WM, SAID-AL AHL H, Tkachenko KG. Bioactive constituents of Buddleja SPP., and their therapeutic potentials. Journal of Biochemistry International. 2022; 9(1):1–11. doi: 10.56557/jobi/2022/v9i17388
7. Wei S, Liu X, Hasan KMF, Peng Y, Xie J, Chen S, et al. Extraction and Purification of Flavonoids from Buddleja officinalis Maxim and Their Attenuation of H2O2-Induced Cell Injury by Modulating Oxidative Stress and Autophagy. Molecules [Internet]. 2022 Dec 1 [cited 2025 Mar 20];27(24):8985. Available from: https://www.mdpi.com/1420-3049/27/24/8985/htm
8. López Hernández OD, Silva Ordoñez MDP, Hernández Navarro Y. Recuperación de polifenoles a partir de residuos del proceso de fabricación de pulpa de mortiño. Revista de Investigación Talentos. 2022;9(2):65–76. doi: 10.33789/talentos.9.2.170
9. Carneiro SB, Kreutz T, Limberger RP, Teixeira HF, da Veiga Júnior VF, Koester LS. Piper aduncum Essential Oil Rich in Dillapiole: Development of Hydrogel-Thickened Nanoemulsion and Nanostructured Lipid Carrier Intended for Skin Delivery. Pharmaceutics. 2022;14(11):2525. doi: 10.3390/pharmaceutics14112525
10. Medda S, Fadda A, Dessena L, Mulas M. Quantification of total phenols, tannins, anthocyanins content in Myrtus communis L. And antioxidant activity evaluation in function of plant development stages and altitude of origin site. Agronomy. 2021;11(6):1059. doi: 10.3390/agronomy11061059
11. Francik S, Francik R, Sadowska U, Bystrowska B, Zawiślak A, Knapczyk A, et al. Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves. Materials (Basel). 2020;13(24):5811. doi: 10.3390/ma13245811
12. Rosero S, Del Pozo F, Simbaña W, Álvarez M, Quinteros MF, Carrillo W, et al. Polyphenols and Flavonoids Composition, Anti-Inflammatory and Antioxidant Properties of Andean Baccharis macrantha Extracts. Plants (Basel). 2022;11(12):1555. doi: 10.3390/plants11121555
13. Bobo-García G, Davidov-Pardo G, Arroqui C, Vírseda P, Marín-Arroyo MR, Navarro M. Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. J Sci Food Agric. 2015;95(1):204–9. doi: 10.1002/jsfa.6706
14. Gǎlbǎu CŞ, Irimie M, Neculau AE, Dima L, Silva LP da, Vârciu M, et al. The Potential of Plant Extracts Used in Cosmetic Product Applications—Antioxidants Delivery and Mechanism of Actions. Antioxidants. 2024;13(11):1425. doi: 10.3390/antiox13111425
15. Yarleque-Chocas M, Dorregaray-Llerena F, Yarleque-Chocas A, Gonzales-Chavesta C, Yarleque-Chocas M, Dorregaray-Llerena F, et al. Actividad antiinflamatoria in vitro de Plantago major L. y Piper aduncum L. sobre la fosfolipasa A2 del veneno de la serpiente Lachesis muta muta. Rev Peru Med Exp Salud Pública. 2023;40(3):325–32
16. Tufinio Miranda K, Ames Canchaya H, Vergara Sotomayor A, Fukusaki Yoshizawa A, Paucar Cuba K. Determinación de la actividad antioxidante de extractos de hojas de Buddleja Inkana, Oreocallis Grandiflora y Chuquiraga Spinosa. Revista de la Sociedad Química del Perú. 2021;87(2):107-119. doi: 10.37761/rsqp.v87i2.343
17. Oprică L, Ivan M, Grigore MN, Zamfirache MM. Antioxidant Activity of Plantago Species in Vegetative and Flowering Stages. Iran J Public Health. 2015;44(1):142-144
18. Samuelsen AB. The traditional uses, chemical constituents and biological activities of Plantago major L. A review. J Ethnopharmacol. 2000;71(1–2):1–21. doi: 10.1016/s0378-8741(00)00212-9
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