Antibacterial screening and GC-MS profile of leave and stem fractions of Calotropis procera (Linn).

Authors

  • Asoso Oluwakemi-Sola
  • Oladunmoye Muftau-Kolawole
  • Ogundare Ayodele Oluyemisi

DOI:

https://doi.org/10.29076/issn.2602-8360vol2iss2.2018pp19-25p

Abstract

Traditional medicine also known as Indigenous or folk medicine comprises of knowledge systems that developed over generations within various societies before the era of modern medicine. The column chromatography was used to collect the crude fractions; solvents like petroleum ether, chloroform and methanol were used. The antibacterial activities of the crude fractions of Calotropis procera (leaf and stem) were evaluated in this study using some selected microorganisms like Escherichia coli, Shigella dysenteriae
ATCC 24162, Salmonella typhi and Klebsiella pneumoniae ATCC 34089, whereby the plant was extracted using
(acetone, methanol and aqueous). The paper disc method was used after which the zone of inhibition around the discs was estimated. The results showed that in the leaf of the plant; E. coli with aqueous-methanol fraction and K. pneumoniae ATCC 34089 with acetone-petroleum ether fraction had the highest yield of 30mm respectively while in stem; K. pneumoniae ATCC 34089 with methanol-methanol fraction had the highest yield of 25mm. The structural elucidation of the bioactive compounds in the extracts were evaluated using GC-MS which reveals the chemical compounds like phenol, methyl palmitate, phthalic acid, 9-ctadecenoic acid and other compounds known for valuable antimicrobial, biological activities and antioxidant properties. The leaves and the stem are having good chemical compounds that can be responsible for the antimicrobial property observed.

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Published

2018-07-11

Issue

Vol. 2 No. 2 (2018): JUNIO-NOVIEMBRE 2018

Section

ARTICULOS

License

Todos los artículos de la Revista FACSalud UNEMI son difundidos bajo licencia internacional Creative Commons Reconocimiento-NoComercial-SinObrasDerivadas 4.0.

Los autores mantienen los derechos de autor, y, por lo tanto, son libres de compartir, copiar, distribuir, ejecutar y comunicar públicamente la obra bajo las condiciones siguientes: Reconocer los créditos de la obra especificada por el autor e indicar si se realizaron cambios (puede hacerlo de cualquier forma razonable, pero no de una manera que sugiera que el autor respalda el uso que hace de su obra. No utilizar la obra para fines comerciales. En caso de remezcla, transformación o desarrollo, no puede distribuirse el material modificado.

How to Cite

Antibacterial screening and GC-MS profile of leave and stem fractions of Calotropis procera (Linn). (2018). FACSALUD-UNEMI, 2(2), 19-25. https://doi.org/10.29076/issn.2602-8360vol2iss2.2018pp19-25p