Hidrólisis enzimática de polvillo de arroz para la producción de etanol de segunda generación

Palabras clave: Azucares reductores, Bioconversión, Celulasas, Cromatografía de Gases, Saccharomyces cerevisiae, etanol

Resumen

El objetivo de la presente investigación fue hidrolizar el polvillo de arroz a azucares reductores y mediante fermentación alcohólica obtener bioetanol. Se prepararon nueve soluciones de polvillo de arroz molido (8 %, 13 % y 18 %) y agua purificada e inoculados con conidios de Trichoderma spp en concentraciones (0,2 g/L, 0,4 g/L y 0,6 g/L), los cuales se incubaron a 30°C por 144 horas.   Mediante el método DNS (3, 5 ácido di nitrosalisilico), se cuantificó los azúcares reductores: tratamiento A: 4,32 ± 0,08%, B: 5,27 ± 0,07%, C: 5,10 ± 0,01%, D: 5,53 ± 0,12%, E: 8,24 ± 0,06%, F: 6,37 ± 0,07%, G: 5,13 ± 0,02%, H: 7,19 ± 0,26%, I: 9,69 ± 0,18% y el etanol mediante cromatografía de gases. El tratamiento que presentó mayor porcentaje de bioconversión fue el tratamiento I (18 % y 0,6 g/L de inóculo), donde la concentración de glucosa, fue 9,88%, y el rendimiento de etanol fue de 5° GL. En conclusión, es posible hidrolizar los carbohidratos totales presente en el polvillo de arroz en azucares reductores y posterior fermentación alcohólica.

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Publicado
2020-01-09
Cómo citar
Ayala-Armijos, J., Machuca-Loja, G., Benítez Castrillón, P., & Alvarado-Cáceres, J. (2020). Hidrólisis enzimática de polvillo de arroz para la producción de etanol de segunda generación . CIENCIA UNEMI, 13(32), 41-50. Recuperado a partir de http://ojs.unemi.edu.ec/index.php/cienciaunemi/article/view/1015
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Artículos Científicos