Queratina a partir de la hidrólisis enzimática de harina de plumas de pollo, utilizando queratinasas producidas por Bacillus subtilis / Getting keratin from feather meal using keratinases produced by Bacillus subtilis
DOI:
https://doi.org/10.29076/issn.2528-7737vol9iss20.2016pp50-58pAbstract
El objetivo de la presente investigación fue hidrolizar harina de plumas de pollo para la obtención de queratina. El diseño metodológico de la investigación fue experimental; se realizaron cuatro fermentaciones correspondientes a un diseño experimental completamente al azar, resultantes de considerar factores [concentración de sustrato (18 g/L y 23 g/L) y concentración del inóculo (2 y 3 g/L), dos niveles para cada factor]. La determinación de la concentración de nitrógeno total se efectuó mediante espectrofotometría UV-Visibles, el método utilizado fue la digestión de persulfato de potasio (K2S2O8), la concentración máxima de nitrógeno se alcanzó en el tratamiento 2 (1,5 %), lo cual multiplicado por el factor proteico 6,25 dio un porcentaje de proteína de 9,4% de queratina en el hidrolizado, presentando diferencia significativa (p<0,05) del resto de tratamientos, al cabo de 24 horas de retención hidráulica. En conclusión, es posible la hidrólisis enzimática de harina de plumas de pollo utilizando el Bacillus subtilis como productor de proteasas.
ABSTRACT
The objective of this research was hydrolyzed feather meal of chicken for the production of keratin. The methodological research design was experimental; four fermentations, corresponding to a completely randomized experimental design, resulting from considering factors [substrate concentration (18 g / L and 23 g / L) and concentration of inoculum (2 and 3 g / L), two levels was performed for each factor]. Determining the total nitrogen concentration was performed by UV-Visible spectrophotometry, the method used is the persulfate potassium digestion (K2S2O8), the maximum concentration of nitrogen is reached in the treatment 2 (1.5%) which multiplied by the factor protein 6.25 gives a percentage of 9.4% protein in the hydrolyzed keratin, showing significant difference (p<0.05) from other treatments at 24 hours hydraulic retention. In conclusion the enzymatic hydrolysis of chicken feather meal is possible using the Bacillus subtilis as a producer of proteases.
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