Antimicrobial activity of hydroalcoholic extracts of leaves of two varieties of Mangifera Indica L
DOI:
https://doi.org/10.29076/issn.2528-7737vol13iss32.2020pp69-77pKeywords:
antimicrobial activity, Kirby Bauer, Mangifera indica LAbstract
The mango (Mangifera indica L) occupies an important place within Ecuadorian export products, representing a source of bioactive compounds the effect of which is dependent on its variety, organ, extraction method and the solvent used. The objective of this work was to evaluate the antimicrobial activity of hydroalcoholic extracts (90% and 50%) of the Tommy Atkins variety and hydroalcoholic extract (50%) of the Edward variety, selected for their high content of phenolic compounds, published previously. The extracts were taken by maceration, digestion and ultrasound. Antimicrobial activity was evaluated using the modified Kirby Bauer and Kirby Bauer methods; the strains used were Pseudomonas aeuroginosa (ATCC 27853), Staphylococcus aureus (ATCC 29213), Salmonella Typhimurium (ATCC 14028), Escherichia coli (ATCC 25922) and Enterococcus faecalis (ATCC 29212), the suspensions being adjusted to 0.5 turbidity on the McFarland scale. A strong antimicrobial activity was found against P. aeruginosa and S. aureus; the Tommy Atkins variety extracts showed the strongest antimicrobial activity, finding inhibition halos between 10 and 15 mm depending on the bacteria. The modified Kirby Bauer method showed the best effectiveness. It is concluded that all the studied strains showed sensitivity to the extracts, with S. aureus and P. aeruginosa being the most sensitive.
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