Evaluation of waste extracts of grapefruit (Citrus paradisi) as a bioactive substance for the formulation of a disinfectant for fresh foods
DOI:
https://doi.org/10.29076/issn.2528-7737vol13iss34.2020pp28-33pKeywords:
grapefruit, Citrus paradisi, antimicrobialAbstract
The search for natural antimicrobials that can be used as disinfection products for fresh fruits and vegetables is a necessity for consumers and the minimally processed food industry. In Ecuador, products are used for this purpose whose raw materials are imported, for this reason it was proposed to evaluate a bioactive substance from the waste of the national grapefruit production. The seeds, albedo (mesocarp) and peel (exocarp) were were subjected to extraction processes by Soxhlet, using ethanol as solvent. For each extract, its antimicrobial activity was evaluated by the modified Kirby-Bauer diffusion method (wells) against different pathogens: Pseudomona aeruginosa, Staphylococcus aureus, Escherichia coli and Rhizopus stoleiner. The albedo and shell extracts showed antibacterial activity against E. coli and S. aureus, while the seed extract showed no sensitivity against any of the micro-organisms, at the concentration tested. Based on these results, different mixtures of the bioactive extracts were prepared, maintaining antibacterial activity and achieving antifungal activity against R. stoleiner. The latter result was superior to that obtained for the evaluation of a commercial product with grapefruit seed active ingredient.
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