In vitro effect of fungicides for the control of Colletotrichum SPP. In fruit trees Manabí - Ecuador
Abstract
Anthracnose caused by Colletotrichum spp causes one of the most limiting diseases in the production of Passiflora edulis and Carica papaya in the province of Manabí, Ecuador. The objective of this research was to determine the pathogenicity of Colletotrichum isolates in passion fruit and papaya fruits, as well as their in vitro sensitivity to fungicides. The experiments were carried out in the Molecular Biology Laboratory of the Escuela Superior Politécnica Agropecuaria de Manabí. The healthy fruits of passion fruit, variety INIAP 2009 and papaya variety Hawaiian, were inoculated by collecting fungal mycelium fragments with the tip of a sterilized wooden stick. For the in vitro sensitivity of fungicides, a completely randomized experimental design was used, 8 commercial fungicide treatments, with 4 repetitions and the comparison of means was carried out with the Tukey test (P <0.05). All the isolates were pathogenic in passion fruit and papaya respectively, they caused characteristic anthracnose symptoms 4 to 5 days after inoculation. The fungicides tebuconazole, propiconazole, difenoconazole, benomyl, carbendazim and azoxystrobin totally inhibited the mycelial growth of the fungus, while chlorothalonil and copper sulfate partially decreased the development of Colletotrichum spp. Systemic fungicides were shown to be effective in the in vitro control of Colletotrichum spp. Isolated from passion fruit and papaya.Downloads
References
Abbott, y Walter S. (1925). A method of computing the effectiveness of an insecticide. J. econ. Entomol 18(2):265-67.
Atghia, O., Alizadeh, A., Fotouhifar, K., Damm, U., Stukenbrock, E., y Javan-Nikkhah, M. (2015). First Report of Colletotrichum fructicola as the causal agent of Anthracnose on Common Bean and Cowpea. Mycologia Iranica, 2(2), 139-140. doi: 10.22043/mi.2015.19966
Bailey, J. (1992). Colletotrichum; biology, pathology and control.
Barnett, H. y Hunter, B. (1998). Illustrated genera of imperfect fungi. St. Paul, Minnesota, USA: The American Phytopathological Society.
Begum, S., Devi, N., Marak, T., Nath, P. y Saha, J. (2015). In Vitro Efficacy of Some Commercial Fungicides Against Colletotrichum capsici, the Causal Agent of Anthracnose of Chilli. Environment & Ecology 33 (4B): 1863—1866
Castaño, Jairo. (1994). Guía para el diagnóstico y control de enfermedades en cultivos de importancia económica.
Eizenga, G., Lee, F. y Rutger, J. (2002). Screening Oryza species plants for rice sheath blight resistance. Plant Disease 86(7):808-12.
French, ER, y TH Teddy. (1980). Métodos de investigación fitopatológica. San José, Costa Rica, IICA». CATIE.
Gaviria, V., Patiño, L. y Saldarriaga, A. (2013). Evaluación in vitro de fungicidas comerciales para el controlde Colletotrichum spp., en mora de castilla. Ciencia y Tecnología Agropecuaria, 14(1), 67-75.
García, R., Cruz, I., Osuna, A. y Marquez, I. (2020). First Report of Carica papaya Fruit Anthracnose Caused by Colletotrichum plurivorum in Mexico. Plant disease. 104:2, 589-589.
Han, Y. C., Zeng, X. G., Xiang, F. Y., Zhang, Q. H., Guo, C., Chen, F.Y. & Gu Y. Chen. (2018). Carbendazim sensitivity in populations of Colletotrichum gloeosporioides complex infecting strawberry and yams in Hubei Province of China. Journal of Integrative Agriculture, 17(6): 1391-1400. DOI: https://doi.org/10.1016/S2095-3119(17)61854-9.
Hyde, K., Lei C., McKenzie, E., Yang, Y., Zhang, J., y Prihastuti, H. (2009). Colletotrichum: a catalogue of confusion. Fungal Diversity 39(1):1-17.
Kim, Ki Deok, BJ Oh, y JM Yang. (1999). Differential interactions of a Colletotrichum gloeosporioides isolate with green and red pepper fruits. Phytoparasitica 27(2):97-106.
Kongtragoul, P., Nalumpang, S., Miyamoto, Y., Izumi, Y. y Akimitsu, K. (2011). Mutación en el codón 198 del gen Tub2 para la resistencia a carbendazim en Colletotrichum gloeosporioides que causa la antracnosis del mango en Tailandia. Journal of Plant Protection Research, 51 (4), 377-384.
Kumar, V., Gupta, V., Babu, A., Mishra, R., Thiagarajan, V. y Datta, R (2001). Surface ultrastructural studies on penetration and infection process of Colletotrichum gloeosporioides on mulberry leaf causing black spot disease. Journal of Phytopathology 149(11‐12):629-33.
Landero, N., Lara, F., Andrade P., Aguilar, L., y Aguado, G. (2016). Alternativas para el control de Colletotrichum spp. Revista mexicana de ciencias agrícolas, 7(5), 1189-1198. Recuperado en 18 de septiembre de 2020, de http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-09342016000501189&lng=es&tlng=es.
Latunde‐Dada, O. y Akinwunmi O. (2001). Colletotrichum: tales of forcible entry, stealth, transient confinement and breakout. Molecular plant pathology 2(4):187-98.
Leslie, J. y Summerell, B. (2008). The Fusarium laboratory manual. John Wiley & Sons.
Marquez, I., Cruz, I., Ley, N., Carrillo, A., Osuna, A., y García, R. (2018). First Report of Carica papaya Fruit Anthracnose Caused by Colletotrichum fructicola in Mexico. Plant disease.102: 12. 2649-2649doi.org/10.1094/PDIS-05-18-0736-PDN
Monteon Ojeda, Abraham, Mora Aguilera, José Antonio, Villegas Monter, Ángel, Nava Diaz, Cristian, Hernández Castro, Elías, Otero-Colina, Gabriel, & Hernández Morales, Javier. (2012). Temporal analysis and fungicide management strategies to control mango anthracnose epidemics in Guerrero, Mexico. Tropical Plant Pathology, 37(6), 375-385. https://doi.org/10.1590/S1982-56762012000600001
Moral, J., Xaviér, C., Roca, L. F., Romero, J., Moreda, W. y Trapero, A. (2014). La antracnosis del olivo y su efecto en la calidad del aceite. Grasas y aceites, 65(2).
Nakaune R, Nakano M. (2007). Benomyl resistance of Colletotrichum acutatum is caused by enhanced expression of beta-tubulin 1 gene regulated by putative leucine zipper protein CaBEN1. Fungal Genet Biol. (12):1324-35. doi: 10.1016/j.fgb.2007.03.007. 0. PMID: 17507270.
Sreenivasaprasad, S., y Pedro Talhinhas. (2005). Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Molecular plant pathology 6(4):361-78.
Sundravadana, S., Alice, D., Kuttalam, S., y Samiyappan, R. (2007). Efficacy of azoxystrobin on Colletotrichum gloeosporiodes penz growth and on controlling mango anthracnose. Journal of Agricultural and Biological Science 2 (3): 10-15.
Zhou, Y., Xu, J., Zhu, Y., Duan, Y., y Zhou, M. (2016). Mechanism of Action of the Benzimidazole Fungicide on Fusarium graminearum: Interfering with Polymerization of Monomeric Tubulin But Not Polymerized Microtubule. Phytopathology 106:8, 807-813.
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